Standards Testing for Gluten Test Kits

Authored by: Ryan Alvarez-Cohen
Published on: Sept 20, 2014

Standard_Test_Gliadin_Samp.JPG

In order to scientifically assess the credibility of test results, FTN generated standards that allow the quantification of scientific controls. For example, the ELISA kits used for testing allergens, such as gluten, need to undergo standards testing in order to confirm the reliability of test results.

Standards testing is a two-step process. First, samples are prepared with known quantities of the substance to be tested (e.g. 20 PPM of gluten). Then these samples are tested with the product to confirm whether the product performs reliably. 

FTN performed rigorous standards testing on the ELISA kits from the supplier, Glutentox. As a first step, we acquired purified gliadin (a gluten peptide without impurities). Gliadin is not soluble in water, therefore in order to prepare liquid samples to test as standards, a surfactant-based method to solubilize the gliadin in water also had to be developed (see figure on the right).

After establishing solubility in the solution, a titration of three liquid samples was prepared with 1, 5 and 20 PPM. As shown in the figures below, the ELISA kits performed successfully on each of the 3 samples.

Figure 1:
20 PPM sample
tested to 20 PPM
shows positive test result
Standard_Test_20_PPM_Samp_20_PPM_Test.JPG


Figure 2:
5 PPM sample
tested to 20 PPM
shows negative test result
Standard_Test_5_PPM_Samp_20_PPM_Test.JPG

 

Figure 3:
5 PPM sample
tested to 5 PPM
shows positive test result
Standard_Test_5_PPM_Samp_5_PPM_Test.JPG

 

Figure 4:
1 PPM sample
tested to 5 PPM
shows negative test result
(but has faint red line indicating some gliaden)
Standard_Test_1_PPM_Samp_5_PPM_Test.JPG